Resumen
A comprehensive ecotoxicological assessment was carried out with Degussa VP nano TiO2 suspension applying a bioluminescent bacterium (Aliivibrio fischeri), algae (Pseudokirchneriella subcapitata, Scenedesmus subspicatus and Chlorella vulgaris), a protozoon (Tetrahymena pyriformis), the water flea (Daphnia magna) and an aquatic macrophyte, Lemna minor. TiO2 nanoparticles were toxic in the set of the conducted tests, but the toxicity level varied with the organisms and endpoints. According to our results the concentrations, the duration and the mechanisms of exposure are contributing factors to the toxicity of nanoparticles. The Tetrahymena phagocytic activity, the Daphnia heartbeat rate and the Lemna total chlorophyll content as ecotoxicity endpoints showed outstanding sensitivity. These organisms showed significant behavioural and physiological changes when exposed to low TiO2nanoparticle concentrations (0.1 and 0.05 µg/L) considered to be lower than the predicted environmental concentration in surface waters. These results reveal the importance of behavioural and physiological assays in assessing the impact of nanoparticles and indicate that nanosized TiO2 may pose risks to the aquatic ecosystem.